Eppendorf Research plus single-channel pipette, fixed, 20 µL, light grey, 3124000032

A fixed-volume pipette has only one value, and that is exactly what its usefulness is based on: the Eppendorf Research plus for 20 µL always dispenses this volume. It cannot be adjusted and therefore cannot be changed by accident. The pipette is mechanical, works with an air cushion and is fully autoclavable. The colour code light grey indicates the matching tip, namely epT.I.P.S. 0.5 to 20 µL L.

When a fixed volume makes sense

A fixed value is worthwhile for work steps that never change: the same addition to every preparation, the same amount of buffer in every vessel. Anyone who switches between several volumes is more flexible with an adjustable-volume pipette. Anyone who performs the same step every day saves the setting with the fixed-volume pipette.

Colour code and tips

According to Eppendorf, the colour of the pipette corresponds to the colour of the matching epT.I.P.S. For this pipette, the tip epT.I.P.S. 0.5 to 20 µL L belongs to the colour code light grey. Some nominal volumes are available in two colours, which belong to different tips. Colour code and tip should therefore be compared before ordering. The pipette is an open system, and tips from other manufacturers are possible. However, the measurement deviations apply only with original epT.I.P.S.

Spring-loaded cone and adjustment

The tip cone is spring-loaded. A locking ring supplied with the pipette deactivates the spring loading. The pipette can be adjusted temporarily. 5 adjustment seals and an adjustment tool (hex key) are supplied for this purpose. It is possible to return to the factory adjustment without calibration.

Typical applications

• Always the same addition of 20 µL to many vessels
• Standard and control solutions with a fixed preparation volume
• Reaction mixtures in which a reagent is always added in the same amount
• Routine protocols in which the volume is specified
• Workstations where several fixed-volume pipettes stand side by side in the stand

Autoclaving and resistance

The entire pipette may go into the autoclave at 121 °C and 1 bar for 20 minutes. Disinfectants and decontamination agents are wiped off beforehand. The pipette is UV-resistant at 254 nm. According to Eppendorf, chemical resistance is high, and special lower parts are available for aggressive chemicals. The pipette is operated at +5 °C to +40 °C and 10 to 95 % relative humidity. For storage, Eppendorf specifies -5 °C to +45 °C.

Technical details

Measurement deviations

Eppendorf specifies the measurement deviation for the nominal volume of this pipette. According to Eppendorf, the values apply only with original epT.I.P.S. Eppendorf determines them gravimetrically according to ISO 8655, with 10 determinations per volume and water according to ISO 3696. The systematic error describes how far the mean value deviates from the set volume. The random error describes the scatter of the individual values.

Scope of delivery

• Pipette
• Operating instructions
• Certificate
• Adjustment tool (hex key)
• 5 adjustment seals for temporary adjustment
• Pipette grease
• Locking ring for deactivating the spring loading

Pipette tips are not included in the scope of delivery.

Accessories and procurement

epT.I.P.S. and pipette holders from Eppendorf are also available. The procurement service takes care of the suitable version on request.