Eppendorf Research plus single-channel pipette, variable, 0.1–2.5 µL, dark grey, 3123000012

Varying sample volumes within the same volume range require a pipette that can be adjusted quickly and without the need for tools. The Eppendorf Research plus single-channel pipette, in its variable 0.1–2.5 µL version in dark grey, meets precisely this requirement, with an increment of 0.002 µL and a spring-loaded tip cone that reduces the force required to attach the tip. It is designed for workstations where dilution series or different sample volumes occur within the same batch. One example of its use is a dilution series in the sub-microlitre range for sequencing preparation, where the required volume varies slightly from batch to batch.

Typical areas of application

The Eppendorf Research plus 0.1–2.5 µL is suitable, amongst other things, for:

• Dilution series in the sub-microlitre range for sequencing preparation, where the volume varies slightly from one reaction to the next
• qPCR master mixes and the addition of minute quantities of reagents
• Pipetting of primers and probes in molecular biology
• Enzyme additions during restriction digestion

Features and handling

A spring-loaded tip cone on all Research-plus models up to 1 mL ensures that pipette tips can be attached and ejected with minimal effort. The pipette is one of the lightest in the Eppendorf range, which makes a noticeable difference to user comfort during long pipetting runs. The lower section, featuring a Fortron PerfectPiston, can be quickly dismantled without tools for maintenance and cleaning.

Eppendorf provides a user adjustment feature for the Research-plus range, known as secondary adjustment, which allows the pipette to be set for a liquid other than water without permanently altering the factory setting.

Selection guide within the Research-plus range

The volume ranges vary significantly across the variable Research-plus models. The following overview shows neighbouring variable models from the same range:

The appropriate volume for each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

This model uses the dark grey tip range (0.1 to 10 µL), the smallest in the Research plus range, adjacent to the variable 0.5–10 µL model.

A comparison of Research plus, Reference 2 and Xplorer

Within the Eppendorf pipette family, Research plus represents the basic manual model: volume is set via the adjustment wheel, and operation is entirely mechanical, with no display and no battery. Reference 2 is based on the same mechanical principle, but controls the volume via a single button and displays it on a four-digit display with a magnifying window, which saves time, particularly when volumes change frequently. The Xplorer and Xplorer plus feature electronic volume adjustment, with programmable sequences such as dilution or reverse pipetting. For those who mainly pipette fixed volumes or volumes that change infrequently, the Research plus is usually a sufficient and more cost-effective option.

Differentiation within the Research plus range

If the volume range of this pipette does not fully meet your requirements, it is worth looking at neighbouring Research plus models, for example the Eppendorf Research plus single-channel pipette, variable (1–10 mL), 3123000080 Eppendorf Research plus G pipette, single-channel, variable, 2–20 µL, light grey, epT.I.P.S. box, 3123000098. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

Autoclave sterilisation is intended to be carried out at 121 °C, 1 bar overpressure and a holding time of 20 minutes whilst the pipette is fully assembled, without dismantling the lower section; the filter sleeve and protective filter are autoclaved separately. There is no need to re-lubricate the pistons afterwards; however, a gravimetric check of accuracy is still advisable. For storage without transport packaging, the temperature range is –5 to 45 degrees Celsius at 10 to 95 per cent relative humidity; the unit is designed for operation at 5 to 40 degrees Celsius within the same range. For warranty enquiries, please contact the relevant Eppendorf authorised dealer; this excludes wear parts and damage caused by misuse or an unauthorised opening of the upper part.

Fixed volume or variable setting

The advantage of the variable version lies in its ability to adapt to changing volumes within a range without having to change the pipette. This is suitable for dilution series, different sample volumes or protocols involving multiple volume steps. If, on the other hand, the required volume remains constant, a fixed-volume pipette from the same range may be the simpler choice and more robust against misadjustment. For dilution series in the sub-microlitre range, where the required volume varies from sample to sample, the variable setting proves its worth, as a single pipette covers the entire range.

Frequently asked questions

Can the pipette withstand steam sterilisation?
Yes, at 121 degrees Celsius and 1 bar overpressure for 20 minutes, whilst assembled and without first dismantling the lower section. The filter sleeve and protective filter must be placed separately in the autoclave.

Does the pipette need to be recalibrated after autoclaving?
It is not necessary to re-lubricate the pistons after autoclaving. However, a gravimetric accuracy check is still recommended afterwards.

How is the volume set on the variable model?
Via the mechanical adjustment wheel on the pipette head, without a display or electronics. The set value is retained until the next adjustment.

Which pipette tips are compatible with the Eppendorf Research plus 0.1–2.5 µL?
According to the operating instructions: epT.I.P.S. dark grey 34 mm (0.1–10 µL).

Is the pipette an in vitro diagnostic device?
According to the operating instructions, the Research plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper section, and wear and tear on typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

Contents

• 1 Eppendorf Research plus single-channel pipette (0.1–2.5 µL, dark grey)

Customised requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.