Eppendorf Research plus single-channel pipette, variable, 10–100 µL, yellow, 3123000047

Varying sample volumes within the same volume range require a pipette that can be adjusted quickly and without the need for tools. The Eppendorf Research plus single-channel pipette, in its variable 10–100 µL version (yellow), meets precisely this requirement, with 0.1 µL increments and a spring-loaded tip cone that reduces the effort required to attach tips. It is designed for workstations where dilution series or different sample volumes are used in the same batch.

Typical applications

The Eppendorf Research plus 10–100 µL is suitable for, amongst other things:

• Standard procedures in molecular biology
• Dilution series and reagent dispensing in routine laboratories
• Sample aliquoting into reaction vessels
• Preparing reaction mixtures in cell culture

Features and handling

All Research-plus pipettes up to 1 mL are fitted with a spring-loaded tip cone, which reduces the force required when changing tips. The pipette is one of the lightest in the Eppendorf range, which makes a noticeable difference to user comfort during long pipetting runs. The Fortron PerfectPiston sits in a base section that can be dismantled without tools, making it practical for cleaning and maintenance.

For secondary adjustment – i.e. calibration to a liquid other than water or to a custom factory setting – the manufacturer states that Research-plus pipettes feature a user adjustment function that does not overwrite the original factory setting.

Selection guide within the Research-plus range

The volume ranges vary significantly across the variable Research-plus models. The following overview shows neighbouring variable models from the same range:

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

A comparison of Research plus, Reference 2 and Xplorer

Research plus, Reference 2 and Xplorer together form the Eppendorf manual pipette family, but differ significantly in terms of operation. Research plus adjusts the volume mechanically via a setting wheel, without any electronics at all. Reference 2 uses a digital display with one-button operation and is particularly suitable for workstations where volumes change frequently. Xplorer and Xplorer plus pipette electronically and are programmable, with battery operation and, in the Plus version, additional functions such as dilution or sequential dispensing. Research plus, on the other hand, stands out as the lightest of the three ranges and the easiest to handle during continuous use.

Differentiation within the Research plus range

If this pipette’s volume range does not fully meet your requirements, it is worth considering neighbouring Research plus models, for example the Eppendorf Research plus single-channel pipette, variable (1–10 mL), 3123000080 Eppendorf Research plus G pipette, single-channel, variable, 2–20 µL, light grey, epT.I.P.S. box, 3123000098. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

The upper and lower sections can be autoclaved together at 121 °C and 1 bar overpressure for 20 minutes; it is not necessary to dismantle the lower section for this. The filter sleeve and protective filter are autoclaved separately. No re-lubrication is required after autoclaving; a gravimetric accuracy check is recommended afterwards. The pipette is stored without transport packaging at temperatures between –5 and 45 degrees Celsius and relative humidity of 10 to 95 per cent; during operation, the temperature range is 5 to 40 degrees Celsius within the same humidity range. Warranty claims are handled via the relevant Eppendorf authorised partner; claims are excluded for damage resulting from misuse, unauthorised opening of the upper section, and wear and tear on typical wear parts.

Fixed volume or variable setting

Anyone who switches between several volumes within the same range in their day-to-day work – for example, when carrying out dilution series or handling different sample volumes per batch – will find a variable-volume pipette more flexible than using several fixed-volume devices. For a single, recurring value, however, a fixed-volume pipette from the Research-plus range is the better choice, as it prevents the adjustment wheel from being turned in the first place.

Frequently asked questions

Can the pipette withstand steam sterilisation?
Yes, at 121 degrees Celsius and 1 bar overpressure for 20 minutes, whilst assembled and without first dismantling the lower section. The filter sleeve and protective filter must be placed separately in the autoclave.

How is accuracy checked after autoclaving?
Re-lubrication is not required; however, Eppendorf still recommends a gravimetric check after autoclaving to confirm accuracy.

How is the volume set on the variable model?
Via the mechanical adjustment wheel on the pipette head, without a display or electronics. The set value is retained until the next adjustment.

Which pipette tips are compatible with the Eppendorf Research plus 10–100 µL?
According to the epT.I.P.S. operating instructions: yellow 53 mm (2–200 µL).

Is the pipette an in vitro diagnostic?
According to the operating instructions, the Research plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

Contents

• 1 Eppendorf Research plus single-channel pipette (10–100 µL, yellow)

Individual requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.