Eppendorf Research plus single-channel pipette, variable, 2–20 µL, yellow, 3123000039
€279.00
- Variable volume range 2–20 µL
- Increment 0.02 µL
- Colour code: yellow
- Spring-loaded tip cone, requires minimal operating force
- Autoclavable at 121 °C for 20 minutes
- epT.I.P.S. yellow 53 mm (2–200 µL)
Product information "Eppendorf Research plus single-channel pipette, variable, 2–20 µL, yellow, 3123000039"
Varying sample volumes within the same volume range require a pipette that can be adjusted quickly and without the need for tools. The Eppendorf Research plus single-channel pipette, in its variable 2–20 µL version (yellow), meets precisely this need, with 0.02 µL increments and a spring-loaded tip cone that reduces the force required for tip loading. It is designed for workstations where dilution series or different sample volumes are used within the same batch.
Typical applications
The Eppendorf Research plus 2–20 µL is suitable for, amongst other things:
- PCR reactions and dilution series
- Reagent addition in ELISA diagnostics
- Sample preparation for sequencing
- Pipetting small volumes into microtitre plates
Features and handling
A spring-loaded tip cone on all Research plus models up to 1 mL ensures that pipette tips can be attached and ejected with minimal effort. Within the Eppendorf pipette range, the Research plus is one of the lightest models, which is an advantage when carrying out many consecutive pipetting steps. The lower section, featuring a Fortron PerfectPiston, can be quickly dismantled without tools for maintenance and cleaning.
For secondary calibration – i.e. calibration for a liquid other than water or to a custom factory setting – the manufacturer states that Research-plus pipettes feature a user calibration function that does not overwrite the original factory setting.
Selection guide within the Research-plus range
The volume ranges vary significantly across the variable Research-plus models. The following overview shows neighbouring variable models from the same range:
| Volume | Colour code | MPN |
| 1–10 mL | turquoise | 3123000080 |
| 2–20 µL | light grey | 3123000098 |
| 30–300 µL | orange | 3123000101 |
| 100–1000 µL | blue | 3123000063 |
The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.
Comparison of Research plus, Reference 2 and Xplorer
When choosing between Research plus, Reference 2 and Xplorer, the frequency of volume changes is the key deciding factor. Research plus adjusts the volume mechanically via a dial, without a display, which makes the pipette lightweight and robust. Reference 2 displays the volume digitally and can be operated using a single button, which is an advantage where there are multiple users and varying protocols. Xplorer and Xplorer plus handle pipetting electronically, including programmable sequences. For workstations with a clearly defined volume range and no need for programming functions, Research plus is the more streamlined solution.
Differentiation within the Research plus range
If the volume range of this pipette does not fully meet your requirements, it is worth considering neighbouring Research plus models, for example the Eppendorf Research plus single-channel pipette, variable (1–10 mL), 3123000080 Eppendorf Research plus G pipette, single-channel, variable, 2–20 µL, light grey, epT.I.P.S. box, 3123000098. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.
Use, storage and maintenance
Autoclaving is possible whilst the pipette is fully assembled, at 121 °C and 1 bar overpressure for 20 minutes; the lower section remains fitted during this process. The filter sleeve and protective filter are autoclaved separately. There is no need to re-lubricate after autoclaving; a gravimetric check afterwards is recommended. Without transport packaging, the pipette should be stored at between –5 and 45 degrees Celsius and 10 to 95 per cent relative humidity; during operation, it should be kept at between 5 and 40 degrees Celsius within the same humidity range. The relevant Eppendorf authorised partner is responsible for warranty matters, with the exception of wear parts and damage resulting from misuse or unauthorised opening of the upper part.
Fixed volume or variable setting
Anyone who switches between several volumes within the same range in their day-to-day work – for example, when carrying out dilution series or handling different sample volumes per batch – will find a variable-volume pipette more flexible than using several fixed-volume devices. For a single, recurring value, however, a fixed-volume pipette from the Research-plus range is the better choice, as it prevents the adjustment wheel from being turned in the first place.
Frequently asked questions
Is the Eppendorf Research plus 2–20 µL autoclavable?
Yes. The upper and lower parts can be autoclaved together at 121 degrees Celsius and 1 bar overpressure for 20 minutes without the lower part needing to be dismantled. The filter sleeve and protective filter are autoclaved separately.
How is accuracy checked after autoclaving?
Re-lubrication is not required; however, Eppendorf still recommends a gravimetric check after autoclaving to confirm accuracy.
How is the volume set on the variable model?
Via the mechanical adjustment wheel on the pipette head, without a display or electronics. The set value is retained until the next adjustment.
Which pipette tips are compatible with the Eppendorf Research plus 2–20 µL?
According to the epT.I.P.S. operating instructions: yellow 53 mm (2–200 µL).
Is the pipette an in vitro diagnostic device?
According to the operating instructions, the Research plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.
To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.
Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.
What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.
Technical details
| Range | Eppendorf Research plus | |
| Model | variable | |
| Volume | 2–20 µL | |
| Increment | 0.02 µL | |
| Colour code | yellow | |
| Compatible pipette tip | epT.I.P.S. yellow 53 mm (2–200 µL) | |
| Test volume 2 µL | Systematic measurement error 5.0%/0.1 µL | Random measurement deviation 1.5%/0.03 µL |
| Test volume 10 µL | Systematic measurement error: 1.2%/0.12 µL | Random measurement error 0.6%/0.06 µL |
| Test volume 20 µL | Systematic measurement error 1.0%/0.2 µL | Random measurement error 0.3%/0.06 µL |
| Standard | Test in accordance with ISO 8655 |
Contents
- 1 Eppendorf Research plus single-channel pipette (2–20 µL, yellow)
Individual requirements & procurement service
For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.
| Colour: | Yellow |
|---|---|
| Number of channels: | 1-channel |
| Operation: | mechanical |
| Volume range: | 2 µl - 20 µl |
| Volume setting: | variable |