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Eppendorf Research plus single-channel pipette, variable, 20–200 µL, yellow, 3123000055

Product information "Eppendorf Research plus single-channel pipette, variable, 20–200 µL, yellow, 3123000055"

Varying sample volumes within the same volume range require a pipette that can be adjusted quickly and without the need for tools. The Eppendorf Research plus single-channel pipette, in its variable 20–200 µL version (yellow), meets precisely this requirement, with 0.2 µL increments and a spring-loaded tip cone that reduces the force required for tip loading. It is designed for workstations where dilution series or different sample volumes are used within the same batch.

Typical applications

The Eppendorf Research plus 20–200 µL is suitable for, amongst other things:

  • Standard work in molecular biology
  • Dilution series and reagent dispensing in routine laboratories
  • Sample aliquoting into reaction vessels
  • Preparing reaction mixtures in cell culture

Features and handling

For volumes up to 1 mL, Eppendorf’s Research plus range features a spring-loaded tip cone, which makes attaching and ejecting the pipette tip noticeably easier. As the lightest pipette in the Eppendorf range, the Research plus reduces hand strain during prolonged batch work. The lower section with a PerfectPiston piston made of Fortron can be quickly dismantled without tools for maintenance and cleaning.

Eppendorf provides a user adjustment feature for the Research plus range, known as secondary adjustment, which allows the pipette to be set for a liquid other than water without permanently altering the factory setting.

Selection guide within the Research-plus range

The volume ranges vary significantly across the variable Research plus models. The following overview shows neighbouring variable models from the same range:

VolumeColour codeMPN
1–10 mLturquoise3123000080
2–20 µLlight grey3123000098
2–20 µLyellow3123000039
30–300 µLorange3123000101

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

Comparing Research plus, Reference 2 and Xplorer

Anyone choosing between the three Eppendorf manual pipette ranges should first consider the operation. The Research plus is set via a mechanical adjustment wheel, with no electronics and no display, making it robust and low-maintenance. The Reference 2 replaces the adjustment wheel with a single-button operation featuring a digital display, which is practical for laboratories with frequently changing volumes and multiple users sharing the same device. The Xplorer and Xplorer plus go one step further, pipetting electronically programmed sequences, including password protection on the Plus model. For a clearly defined volume range in routine work, the mechanical Research plus range remains the simplest choice.

Differentiation within the Research plus range

If the volume range of this pipette does not fully meet your requirements, it is worth considering neighbouring Research plus models, for example the Eppendorf Research plus single-channel pipette, variable (1–10 mL), 3123000080 and Eppendorf Research plus G pipette, single-channel, variable, 2–20 µL, light grey, epT.I.P.S. box, 3123000098. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

121 °C, 1 bar gauge pressure, 20 minutes: the pipette can be autoclaved whilst fully assembled; the lower section does not need to be dismantled for this, only the filter sleeve and protective filter are placed separately in the autoclave. There is no need to re-lubricate the pistons afterwards, although a gravimetric check is still advisable. Without transport packaging, storage is intended at temperatures between –5 and 45 °C and relative humidity between 10 and 95 %; during operation, the range is 5 to 40 °C with the same humidity range. The relevant Eppendorf authorised dealer is the point of contact for warranty matters, excluding wear parts and damage resulting from misuse or the upper part being opened without authorisation.

Fixed volume or variable setting

Those who switch between several volumes within the same range in their day-to-day work, for example when carrying out dilution series or handling different sample volumes per batch, will find a variable-volume pipette more flexible than using several fixed-volume devices. For a single, recurring value, however, a fixed-volume pipette from the Research-plus range is worthwhile, as it prevents the adjustment wheel from being turned in the first place.

Frequently asked questions

Is the Eppendorf Research plus 20–200 µL autoclavable?
Yes. The upper and lower sections can be autoclaved together at 121 °C and 1 bar gauge pressure for 20 minutes without the lower section needing to be dismantled. The filter sleeve and protective filter are autoclaved separately.

Does the pipette need to be readjusted after autoclaving?
It is not necessary to re-lubricate the pistons after autoclaving. However, a gravimetric accuracy check is still recommended afterwards.

How is the volume set on the variable model?
Via the mechanical adjustment wheel on the pipette head, without a display or electronics. The set value is retained until the next adjustment.

Which pipette tips are compatible with the Eppendorf Research plus 20–200 µL?
According to the operating instructions: epT.I.P.S. yellow 53 mm (2–200 µL).

Is the pipette an in vitro diagnostic device?
No. Eppendorf lists the Research plus as a pipette for general laboratory use; it does not carry CE-IVD marking. Eppendorf discontinued the earlier IVD version under Directive 98/79/EC in early 2022, when Regulation (EU) 2017/746 replaced the Directive. According to the manufacturer, the pipette is technically unchanged and may be used for general laboratory applications, including IVD applications.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear on typical wearing parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary adjustment is provided for the Research-plus range. Details are set out in a separate Eppendorf adjustment document.

Technical details

RangeEppendorf Research plus
Modelvariable
Volume20–200 µL
Increment0.2 µL
Colour codeyellow
Compatible pipette tipepT.I.P.S. yellow 53 mm (2–200 µL)
Test volume 20 µLSystematic measurement error 2.5 %/0.5 µLRandom measurement error 0.7 %/0.14 µL
Test volume 100 µLSystematic measurement error 1.0 %/1.0 µLRandom measurement error 0.3 %/0.3 µL
Test volume 200 µLSystematic measurement error 0.6 %/1.2 µLRandom measurement error 0.2 %/0.4 µL
StandardTesting according to ISO 8655

Scope of delivery

  • 1 Eppendorf Research plus single-channel pipette (20–200 µL, yellow)

Individual requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, we provide support via our procurement service.

Colour: Yellow
Number of channels: 1-channel
Operation: mechanical
Volume range: 20 µl - 200 µl
Volume setting: variable