Eppendorf Research plus single-channel pipette, fixed, 20 µL, yellow, 3124000040

Anyone who repeatedly pipettes the same volume does not need a variable setting, but rather a reliable preset that cannot be accidentally adjusted. The yellow Eppendorf Research plus 20 µL is designed precisely for this purpose: a fixed volume of 20 µL, a spring-loaded tip cone for effortless tip attachment, and a low weight within the Eppendorf pipette range. The pipette is suitable for routine procedures where the same volume is dispensed over many repetitions. One example is the addition of loading buffer prior to gel electrophoresis, where the same volume is dispensed into each sample well and a variable setting would offer no additional benefit.

Typical applications

The Eppendorf Research plus 20 µL is suitable, amongst other things, for:

• Adding loading buffer prior to gel electrophoresis, where the same volume is dispensed into each sample well
• PCR reactions and dilution series
• Reagent dispensing in ELISA diagnostics
• Sample preparation for sequencing

Features and handling

For volumes up to 1 mL, Eppendorf’s Research-plus range features a spring-loaded tip cone, which makes attaching and ejecting the pipette tip noticeably easier. The pipette is one of the lightest in the Eppendorf range, which makes a noticeable difference to user comfort during long pipetting runs. The lower section, featuring a Fortron PerfectPiston, can be quickly dismantled without tools for maintenance and cleaning.

Eppendorf provides a user adjustment feature for the Research-plus range, known as secondary adjustment, which allows the pipette to be set for a liquid other than water without permanently altering the factory setting.

Selection guide within the Research-plus range

Within the fixed-preset Research-plus models, there are several volumes to choose from. The following overview shows adjacent fixed-volume versions from the same range, each with its own colour code and manufacturer’s number:

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

For adding loading buffer, the yellow tip range (2 to 200 µL) is suitable, which also covers the variable 2–20 µL and 20–200 µL versions in the range.

A comparison of Research plus, Reference 2 and Xplorer

Research plus, Reference 2 and Xplorer together form the Eppendorf manual pipette family, but differ significantly in terms of operation. Research plus adjusts the volume mechanically via a setting wheel, without any electronics at all. Reference 2, on the other hand, uses a digital display with one-button operation and is particularly suitable for workstations where volumes change frequently. Xplorer and Xplorer plus feature electronic, programmable pipetting, are battery-operated and, in the Plus version, offer additional functions such as dilution or sequential dispensing. Research plus, on the other hand, stands out as the lightest of the three ranges and the easiest to handle during continuous use.

Differentiation within the Research plus range

If this pipette’s volume range does not fully meet your requirements, it is worth looking at neighbouring Research plus models, for example the Eppendorf Research plus G pipette, single-channel, fixed, 200 µL, blue, 3124000091 Eppendorf Research plus G pipette, single-channel, fixed, 50 µL, yellow, 3124000067. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

Autoclaving is possible whilst the pipette is fully assembled, at 121 °C and 1 bar overpressure for 20 minutes; the lower section remains fitted during this process. The filter sleeve and protective filter are autoclaved separately. Re-lubrication after autoclaving is not required; a gravimetric check afterwards is recommended. Without transport packaging, the pipette should be stored at between –5 and 45 degrees Celsius and 10 to 95 per cent relative humidity; during operation, it should be kept at between 5 and 40 degrees Celsius within the same humidity range. The relevant Eppendorf authorised partner is responsible for warranty matters, excluding wear parts and damage resulting from misuse or unauthorised opening of the upper part.

Fixed volume or variable setting

Fixed-volume pipettes are ideal for work steps that always require the same volume, for example, routine dispensing in diagnostics or standardised sample preparation. As there is no adjustment wheel, there is no need to check the set value before each use, which saves time and prevents operating errors caused by accidental turning. For workstations with varying volume requirements, the Research-plus range offers suitable variable-volume models. When loading buffers prior to gel electrophoresis, the volume is strictly specified by the protocol; a fixed preset eliminates the need to check the adjustment wheel before each individual sample pocket.

Frequently Asked Questions

Can the pipette withstand steam sterilisation?
Yes, at 121 degrees Celsius and 1 bar overpressure for 20 minutes, whilst assembled and without first dismantling the lower section. The filter sleeve and protective filter must be placed separately in the autoclave.

Is re-lubrication necessary after autoclaving?
No, according to the manufacturer, there is no need to re-lubricate the pistons after autoclaving. However, a gravimetric check of accuracy afterwards is recommended.

Can the volume of the 20 µL version be adjusted?
No, this version has a fixed preset volume of 20 µL and no adjustment wheel for selecting the volume. If you require different volumes within the same range, you will find the appropriate variable models in the Research plus range.

Which pipette tips are compatible with the Eppendorf Research plus 20 µL?
According to the operating instructions: epT.I.P.S. yellow 53 mm (2–200 µL).

Is the pipette an in vitro diagnostic?
According to the operating instructions, the Research-plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

Contents

• 1 Eppendorf Research plus single-channel pipette (20 µL, yellow)

Individual requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, we provide support via our own procurement service.