Eppendorf Research plus single-channel pipette, fixed, 200 µL, blue, 3124000091

Anyone who repeatedly pipettes the same volume does not need a variable setting, but rather a reliable preset that cannot be accidentally adjusted. The Eppendorf Research plus 200 µL in blue is designed precisely for this purpose: a fixed volume of 200 µL, a spring-loaded tip cone for effortless tip attachment, and a low weight within the Eppendorf pipette range. The pipette is suitable for routine procedures where the same volume is dispensed over many repetitions.

Typical applications

The Eppendorf Research plus 200 µL is suitable for, amongst other things:

• Standard procedures in molecular biology
• Dilution series and reagent dispensing in routine laboratories
• Sample aliquoting into reaction vessels
• Preparing reaction mixtures in cell culture

Features and handling

For volumes up to 1 mL, Eppendorf’s Research plus range features a spring-loaded tip cone, which makes attaching and ejecting the pipette tip noticeably easier. Within the Eppendorf pipette family, Research plus is one of the lightest models, an advantage when carrying out many consecutive pipetting steps. For cleaning and maintenance, the lower section with the Fortron PerfectPiston can be quickly dismantled without the need for tools.

For secondary calibration – i.e. adjustment to a liquid other than water or to a custom factory setting – the manufacturer states that Research plus pipettes feature a user-adjustment function that does not overwrite the original factory setting.

Selection guide within the Research-plus range

Within the fixed-preset Research-plus models, there are several volumes to choose from. The following overview shows adjacent fixed-volume versions from the same range, each with its own colour code and manufacturer’s reference number:

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

Comparison of Research plus, Reference 2 and Xplorer

When choosing between Research plus, Reference 2 and Xplorer, the frequency of volume changes is the key deciding factor. Research plus adjusts the volume mechanically via a dial, without a display, which makes the pipette lightweight and robust. Reference 2 displays the volume digitally and can be operated using a single button, which is an advantage where there are multiple users and varying protocols. Xplorer and Xplorer plus handle pipetting electronically, including programmable sequences. For workstations with a clearly defined volume range and no need for programming functions, Research plus is the more streamlined solution.

Differentiation within the Research plus range

If the volume range of this pipette does not fully meet your requirements, it is worth looking at neighbouring Research plus models, for example the Eppendorf Research plus G pipette, single-channel, fixed, 50 µL yellow, 3124000067, and the Eppendorf Research plus G pipette, single-channel, fixed, 20 µL yellow, 3124000040. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

The pipette can be autoclaved whilst assembled at 121 °C, 1 bar overpressure for 20 minutes, without the need to dismantle the lower section. The filter sleeve and protective filter must be placed in the autoclave separately. Re-lubrication of the pistons is not required afterwards, although a gravimetric check is nevertheless recommended. For storage without packaging, the conditions are –5 to 45 degrees Celsius and 10 to 95 per cent relative humidity; the device is designed for operation at 5 to 40 degrees Celsius within the same humidity range. The warranty is handled via the local Eppendorf authorised partner; wear parts and damage resulting from improper use or unauthorised opening of the upper part are excluded from the warranty.

Fixed volume or variable setting

Fixed-volume pipettes are ideal for tasks that always require the same volume, such as routine dispensing in diagnostics or standardised sample preparation. As there is no adjustment wheel, there is no need to check the set value before each use, which saves time and prevents operating errors caused by accidental turning. For workstations with varying volume requirements, the Research-plus range offers suitable variable-volume models.

Frequently asked questions

Is the Eppendorf Research plus 200 µL autoclavable?
Yes. The upper and lower sections can be autoclaved together at 121 degrees Celsius and 1 bar overpressure for 20 minutes without the lower section needing to be dismantled. The filter sleeve and protective filter are autoclaved separately.

Does the pipette need to be recalibrated after autoclaving?
It is not necessary to re-lubricate the pistons after autoclaving. However, a gravimetric accuracy check is still recommended afterwards.

Can the volume of the 200 µL version be adjusted?
No, this version has a fixed, preset volume of 200 µL and no volume selection dial. If you require different volumes within the same range, you will find suitable variable models in the Research plus range.

Which pipette tips are compatible with the Eppendorf Research plus 200 µL?
According to the operating instructions: epT.I.P.S. blue 71 mm (50–1,000 µL).

Is the pipette an in vitro diagnostic?
According to the operating instructions, the Research-plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

Contents

• 1 Eppendorf Research plus single-channel pipette (200 µL, blue)

Individual requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.