Eppendorf Research plus single-channel pipette, fixed, 50 µL, yellow, 3124000067

Anyone who repeatedly pipettes the same volume does not need a variable setting, but rather a reliable preset that cannot be accidentally adjusted. The Eppendorf Research plus 50 µL in yellow is designed precisely for this purpose: a fixed volume of 50 µL, a spring-loaded tip cone for effortless tip attachment, and a low weight within the Eppendorf pipette range. The pipette is suitable for routine procedures where the same volume is dispensed over many repetitions. One example is the preparation of standard series for calibration, where the same sub-volume is measured several times in succession, step by step in the same quantity.

Typical applications

The Eppendorf Research plus 50 µL is suitable, amongst other things, for:

• Dilution steps in the preparation of standard series, where the same sub-volume is dispensed multiple times
• Standard procedures in molecular biology
• Dilution series and reagent dispensing in routine laboratory work
• Aliquoting samples into reaction vessels

Features and handling

A spring-loaded tip cone on all Research plus models up to 1 mL ensures that pipette tips can be attached and ejected with minimal effort. As the lightest pipette in the Eppendorf pipette range, the Research plus reduces hand strain during prolonged batch work. The lower section, featuring a Fortron PerfectPiston, can be quickly dismantled without tools for maintenance and cleaning.

For secondary adjustment – i.e. calibration to a liquid other than water or to a custom factory setting – the manufacturer states that Research plus pipettes feature a user adjustment function that does not override the original factory setting.

Selection guide within the Research-plus range

Within the fixed-preset Research-plus models, there are several volumes to choose from. The following overview shows adjacent fixed-volume versions from the same range, each with its own colour code and manufacturer’s reference number:

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

This version is part of the yellow tip family (2 to 200 µL), alongside the adjacent fixed-volume models (20 µL, 25 µL, 100 µL and 200 µL) from the same range.

Research plus, Reference 2 and Xplorer compared

Within the Eppendorf pipette range, Research plus represents the basic manual model: the volume is set using the adjustment wheel, and operation is entirely mechanical, with no display and no battery. Reference 2 is based on the same mechanical principle, but controls the volume via a single button and displays it on a four-digit display with a magnifying window, which saves time, particularly when volumes change frequently. The Xplorer and Xplorer plus feature electronic volume adjustment, with programmable sequences such as dilution or reverse pipetting. For those who mainly pipette fixed volumes or volumes that change infrequently, the Research plus is usually a sufficient and more cost-effective option.

Differentiation within the Research plus range

If the volume range of this pipette does not fully meet your requirements, it is worth looking at neighbouring Research plus models, for example the Eppendorf Research plus G pipette, single-channel, fixed, 200 µL, blue, 3124000091 Eppendorf Research plus G pipette, single-channel, fixed, 20 µL, yellow, 3124000040. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

Autoclave sterilisation is intended to be carried out at 121 °C, 1 bar overpressure and a holding time of 20 minutes whilst the pipette is fully assembled, without dismantling the lower section; the filter sleeve and protective filter are autoclaved separately. There is no need to re-lubricate the pistons afterwards, although a gravimetric check of accuracy is nevertheless advisable. For storage without transport packaging, the temperature range is –5 to 45 degrees Celsius at 10 to 95 per cent relative humidity; the unit is designed for operation at 5 to 40 degrees Celsius within the same range. The relevant Eppendorf authorised dealer is responsible for warranty matters, excluding wear parts and damage caused by misuse or an unauthorised opening of the upper part.

Fixed volume or variable setting

A pipette with a fixed preset is ideal wherever the same volume is required over many repetitions, such as in standardised dilution steps or when adding reagents to batch preparations. The absence of an adjustment wheel reduces the risk of accidental misadjustment and speeds up batch work, as there is no longer any need to check the volume value. On the other hand, those who regularly switch between different volumes are better served by a variable Research-plus pipette from the same range. For dilution steps involving a consistent sub-volume per step, the fixed preset reduces the risk of accidentally setting a different volume between two dilution stages.

Frequently asked questions

Is the Eppendorf Research plus 50 µL autoclavable?
Yes. The upper and lower sections can be autoclaved together at 121 degrees Celsius and 1 bar overpressure for 20 minutes without the lower section needing to be dismantled. The filter sleeve and protective filter are autoclaved separately.

Does the pipette need to be recalibrated after autoclaving?
It is not necessary to re-lubricate the pistons after autoclaving. However, a gravimetric accuracy check is still recommended afterwards.

Can the volume of the 50 µL version be adjusted?
No, this version has a fixed preset volume of 50 µL and no volume selection dial. If you require different volumes within the same range, you will find suitable variable models in the Research plus range.

Which pipette tips are compatible with the Eppendorf Research plus 50 µL?
According to the operating instructions: epT.I.P.S. yellow 53 mm (2–200 µL).

Is the pipette an in vitro diagnostic device?
According to the operating instructions, the Research-plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

Contents

• 1 Eppendorf Research plus single-channel pipette (50 µL, yellow)

Individual requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.