IKA S 10 N - 5 G dispersing tool, 5 mm, 0003304000
€1,168.50
% Manufacturer's RRP: €1,558.00 25% below manufacturer's RRP
- Rotor-stator dispersion on a micro-scale
- Volume range: 0.5 to 10 millilitres
- Extremely high shear forces in the shear gap
- Stainless steel and PTFE in contact with the medium
- Thermally autoclavable for absolute sterility
- Sliding bearings prevent thermodynamic dry running
- Manufacturer’s part number (MPN) 0003304000 | EAN 4053411004194
Product information "IKA S 10 N - 5 G dispersing tool, 5 mm, 0003304000"
Rotor-stator kinematics and sub-millimetre shear stress
The physical breakdown of tissues (homogenisation) or the production of highly stable emulsions on a micro-scale requires extremely high-frequency mechanical energy dissipation. The IKA S 10 N - 5 G dispersing tool operates according to the process engineering rotor-stator principle within a working volume of 0.5 to 10 ml. Driven by the T 10 basic drive at up to 30,000 revolutions per minute, the fluid is drawn hydrodynamically into the centre of the rotor and forced outwards by radial centrifugal force. The process-critical stage takes place in the gap, exactly 0.1 mm wide, between the 3.8-mm rotor and the 5-mm stator. The extremely high flow velocities occurring here (peripheral velocities of up to 6.0 m/s) generate enormous mechanical shear forces and localised hydrodynamic cavitation, which irreversibly shatter the cell membranes or droplet geometries in fractions of a millisecond.
Thermodynamic limitations and fluid friction
The application of such massive kinetic energy within a microscopic shear gap inevitably induces massive thermodynamic exothermic heat (frictional heat). As the surface-to-volume ratio is extremely low in microvolumes (e.g. in Eppendorf vials), this thermal energy cannot be passively dissipated. Without external cryogenic cooling (ice bath), the matrix would boil within seconds and thermolabile analytes (such as RNA, DNA or proteins) would denature. The PTFE shaft’s plain bearing is highly chemically resistant; however, it restricts the system to wet grinding only. Dry running without a liquid matrix would mechanically destroy the tribology of the PTFE bearing due to immediate thermal overload.
Material integrity and molecular biological sterility
In PCR trace analysis or forensics, the absolute prevention of molecular cross-contamination (carry-over of foreign nucleic acids) is the paramount metrological principle. The media-contacting profile of the S 10 N - 5 G is made entirely from high-alloy, corrosion-resistant stainless steel (AISI 316L) and PTFE. This monolithic material architecture allows for thorough decontamination using aggressive solvents as well as complete thermal sterilisation in an autoclave (121 °C). To maintain hydrodynamic flow and cavitation in the long term, the microscopically fine slots in the stator must be cleaned with metrological precision in order to physically remove biofilms or polymerisation residues.
Technical details
Fluid mechanics & kinematics
- Processing principle: Rotor-stator shear comminution
- Operating volume range (based on H₂O): 0.5 – 10 ml
- Stator diameter: 5 mm
- Rotor diameter: 3.8 mm
- Gap width (shear zone): 0.1 mm
- Peripheral speed (max. at 30,000 rpm): approx. 6.0 m/s
- Permissible final particle size (suspensions): 5 – 25 µm
- Permissible final particle size (emulsions): 1 – 10 µm
Geometry & system limits
- Minimum/maximum immersion depth: 20 mm / 75 mm
- Shaft length (total): 92 mm
- Permissible viscosity of the medium (max.): 5,000 mPa·s
- Tribological bearing arrangement: plain bearing (wet running only permitted)
Materials Science & Thermodynamics
- Materials in contact with the medium: stainless steel (AISI 316L / 1.4404) and PTFE (polytetrafluoroethylene)
- Operating pH range: pH 2 – 13
- Thermal limitation (maximum operating temperature): 180 °C (short-term/for sterilisation)
- Autoclavability: Yes, fully thermally sterilisable (121 °C / 2 bar)
- Permitted sterilisation methods: Autoclaving, chemical, flame (restricted due to PTFE)
Contents of delivery
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1 x IKA S 10 N - 5 G dispersing tool
(Important note: The ULTRA-TURRAX T 10 basic / standard high-speed drive, which is essential for rotation, is not included in the scope of delivery!)
Individual requirements & accessories
The S 10 N - 5 G is purely a kinetic interface and does not generate any shear forces without the IKA T 10 basic drive system. In analytical molecular biology, the cleaning protocol for microtubes is extremely time-consuming. For high-throughput laboratories (high sample throughput), we therefore strongly recommend purchasing several identical tools to avoid disrupting the analytical workflow during autoclaving cycles. Please contact our procurement service at any time for strategic requirements planning for your dispersion infrastructure.
Fits
The IKA T 10 basic ULTRA-TURRAX is a hand-held disperser for small sample volumes of between 0.5 and 100 millilitres (H₂O). The unit is powered directly from the 230-volt mains supply, requires no battery and is ready for use immediately upon plugging it in. The speed is infinitely variable from 8,000 to 30,000 revolutions per minute, allowing it to process both low-viscosity aqueous samples and thicker emulsions up to 5,000 mPas.Continuously variable speed via the scale displayOn the T 10 basic, the speed is read from a scale on the housing; the battery-powered ‘battery’ version displays it digitally. This is sufficient for a procedure where the same mark can always be selected, and operation remains simple. When plugged in, the unit draws 125 watts, 75 watts of which are delivered to the motor. A duty cycle of 10 minutes followed by a 5-minute pause protects the motor from overheating during longer production runs.Tool change without additional toolsThe arm has a diameter of 8 millimetres and a length of 100 millimetres. The dispersion tool can be removed without any additional tools using the quick-release coupling. Stainless steel dispersion tools are dismantled and cleaned individually for this purpose, whilst the disposable plastic tools, available in two sizes, come ready-assembled from the packaging and are disposed of after use. This reduces the cleaning effort required between two samples and prevents cross-contamination, for example in PCR analysis. No dispersing tool is included in the scope of delivery; it is selected separately to suit the application.Compact and lightweight for manual operationMeasuring 178 millimetres in length, 56 in width and 66 in depth, the T 10 basic fits comfortably in the hand. Weighing 0.61 kilograms, the device can be held for extended periods without causing fatigue; the noise level without an attachment fitted is 65 dB(A).For different mains voltagesIn addition to the standard 230-volt version, IKA also supplies the same unit in 115-volt and 100-volt versions, for countries outside the European mains supply or for branch sites with different mains voltages. The IP 30 protection rating in accordance with DIN EN 60529 and a permissible operating range of between 5 and 40 degrees Celsius at up to 80 per cent relative humidity are sufficient for normal day-to-day laboratory use, although the unit is not designed for use outdoors or in damp rooms.Typical applicationsHomogenisation of small tissue samples prior to further extractionPreparation and fine dispersion of emulsions and suspensions on a micro-scaleSample preparation for PCR analysis using disposable plastic instrumentsRoutine tasks in teaching and training where a robust instrument that does not require battery maintenance is requiredSelection guide: basic or battery?The T 10 basic runs exclusively on mains power and therefore always requires a power socket within reach. For those who wish to use the disperser under a fume cupboard or on the move without a cable, the T 10 battery ULTRA-TURRAX is the battery-powered variant of the same series, albeit with lower motor power and a lower viscosity limit of 200 mPas. For mobile use with suitable accessories, there are also three kits available, such as the T 10 battery Portable Solution or the T 10 battery Tissue Safe Solution with disposable tools for tissue samples.Technical detailsMotor power input / output125 W / 75 WProcessable volume (H₂O)0.5 to 100 mlMax. viscosity5,000 mPasSpeed range8,000 to 30,000 rpm, continuously variableSpeed displayScaleNoise level without tools65 dB(A)Boom diameter / boom length8 mm / 100 mmDuty cycleON 10 mins, OFF 5 minsPermissible ambient temperature / humidity5 to 40 °C / 80 %Protection classIP 30 in accordance with DIN EN 60529Dimensions (W x H x D)56 x 178 x 66 mmWeight0.61 kgPower rating230 V, 50/60 Hz, 125 W (other voltage options: 115 V / 100 V)ContentsT 10 basic ULTRA-TURRAX drive unit, ID No. 0003737000R 200 clamp, ID No. 0003372000A dispersing tool is not included in the scope of delivery and must be selected separately from the range of accessories to suit the application.Regulatory informationThis unit is an electrical appliance within the meaning of the German Electrical and Electronic Equipment Act (ElektroG). The WEEE registration number of the manufacturer, IKA, is DE 66784213. Information on the return of end-of-life equipment can be found on our Environment and Disposal page.Warranty and procurement serviceThe T 10 basic is covered by IKA’s manufacturer’s warranty: 2 years, extended to 3 years upon registration at ika.com/register within 90 days of purchase. LT Laborhandel’s procurement service will assemble suitable dispersing tools, stands or clamps from the T-10 range of accessories.