Eppendorf Research plus single-channel pipette, fixed, 1000 µL, blue, 3124000121

Anyone who repeatedly pipettes the same volume does not need a variable setting, but rather a reliable preset that cannot be accidentally adjusted. The Eppendorf Research plus 1000 µL in blue is designed precisely for this purpose: a fixed volume of 1000 µL, a spring-loaded tip cone for effortless tip attachment, and a low weight within the Eppendorf pipette range. The pipette is suitable for routine procedures in which the same volume is dispensed over many repetitions. A classic example is medium change in cell culture, where the same volume of culture medium is removed from and added back to each culture vessel, day after day, following the same procedure.

Typical applications

The Eppendorf Research plus 1000 µL is suitable for, amongst other things:

• Media changes in cell culture, where the same volume of culture medium is always removed or added to each culture vessel
• Buffer preparations and reagent dispensing in the medium-volume range
• Sample aliquoting and sample preparation
• Filling reaction vessels and cuvettes

Features and handling

For volumes up to 1 mL, Eppendorf’s Research-plus range features a spring-loaded tip cone, which makes attaching and ejecting the pipette tip noticeably easier. The pipette is one of the lightest in the Eppendorf range, which makes a noticeable difference to user comfort during long pipetting runs. For cleaning and maintenance, the lower section with the Fortron PerfectPiston can be quickly dismantled without the need for tools.

If the pipetting result deviates from the expected accuracy – for example, when using a liquid other than water – the pipette can be readjusted via a user adjustment without losing the original factory setting.

Selection guide within the Research-plus range

Several volumes are available within the pre-set Research-plus models. The following overview shows adjacent fixed-volume versions from the same range, each with its own colour code and manufacturer’s number:

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

For medium changes, the blue tip range (50 to 1000 µL) is suitable; this range also includes the 200 µL, 250 µL and 500 µL fixed-volume versions, as well as the variable 100–1000 µL version from the same range.

A comparison of Research plus, Reference 2 and Xplorer

When choosing between Research plus, Reference 2 and Xplorer, the frequency of volume changes is the key deciding factor. Research plus adjusts the volume mechanically via a dial, without a display, which makes the pipette lightweight and robust. Reference 2 displays the volume digitally and is operated via a single button, which is an advantage where there are multiple users and varying protocols. Xplorer and Xplorer plus handle pipetting electronically, including programmable sequences. For workstations with a clearly defined volume range and no need for programming functions, Research plus is the more streamlined solution.

Differentiation within the Research plus range

If the volume range of this pipette does not fully meet your requirements, it is worth looking at neighbouring Research plus models, for example the Eppendorf Research plus G pipette, single-channel, fixed, 200 µL, blue, 3124000091 Eppendorf Research plus G pipette, single-channel, fixed, 50 µL, yellow, 3124000067. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

Sterilisation in an autoclave is intended to be carried out at 121 °C, 1 bar overpressure and a holding time of 20 minutes whilst the pipette is fully assembled, without dismantling the lower section; the filter sleeve and protective filter are autoclaved separately. There is no need to re-lubricate the pistons afterwards, although a gravimetric check of accuracy is nevertheless advisable. For storage without transport packaging, the temperature range is –5 to 45 degrees Celsius at 10 to 95 per cent relative humidity; the unit is designed for operation at 5 to 40 degrees Celsius within the same range. The relevant Eppendorf authorised dealer is responsible for warranty matters, excluding wear parts and damage caused by misuse or an unauthorised opening of the upper part.

Fixed volume or variable setting

Where a pipetting step is always carried out with the same volume – for example, with standardised test kits or recurring dilution steps – a fixed-volume pipette saves time compared with readjusting a variable pipette. At the same time, the risk of accidental adjustment during batch work is reduced. If the required volume varies from project to project, a variable Research-plus pipette from the same range is the more flexible alternative. During daily media changes in cell culture, the volume per culture vessel is specified by the protocol; a fixed preset speeds up batch processing and prevents the dial from being accidentally adjusted.

Frequently Asked Questions

Is the Eppendorf Research plus 1000 µL autoclavable?
Yes. The upper and lower sections can be autoclaved together at 121 degrees Celsius and 1 bar overpressure for 20 minutes without the lower section needing to be dismantled. The filter sleeve and protective filter are autoclaved separately.

Does the pipette need to be recalibrated after autoclaving?
It is not necessary to re-lubricate the pistons after autoclaving. However, a gravimetric accuracy check is still recommended afterwards.

Can the volume of the 1000 µL version be adjusted?
No, this version has a fixed preset volume of 1000 µL and no volume selection dial. If you require different volumes within the same range, you will find suitable variable models in the Research plus range.

Which pipette tips are compatible with the Eppendorf Research plus 1000 µL?
According to the operating instructions: epT.I.P.S. blue 71 mm (50–1000 µL).

Is the pipette an in vitro diagnostic device?
According to the operating instructions, the Research-plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

Contents

• 1 Eppendorf Research plus single-channel pipette (1000 µL, blue)

Individual requirements & procurement service

For suitable pipette tips, spare parts or other volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.