Eppendorf Research plus single-channel pipette, variable, 100–1000 µL, blue, 3123000063

Varying sample volumes within the same volume range require a pipette that can be adjusted quickly and without the need for tools. The Eppendorf Research plus single-channel pipette, in its variable 100–1000 µL blue version, meets precisely this requirement, with 1 µL increments and a spring-loaded tip cone that reduces the force required for tip loading. It is designed for workstations where dilution series or different sample volumes are used within the same batch.

Typical applications

The Eppendorf Research plus 100–1,000 µL is suitable for, amongst other things:

• Media changes in cell culture
• Buffer preparations and reagent dispensing in the medium-volume range
• Sample aliquoting and sample preparation
• Filling reaction vessels and cuvettes

Features and handling

All Research-plus pipettes up to 1 mL are fitted with a spring-loaded tip cone, which reduces the force required for tip changes. Within the Eppendorf pipette range, Research-plus is one of the lightest models, an advantage during many consecutive pipetting steps. For cleaning and maintenance, the lower section with the Fortron PerfectPiston can be quickly disassembled without tools.

Eppendorf provides a user-adjustment feature for the Research-plus range, known as secondary adjustment, which allows the pipette to be set for a liquid other than water without permanently altering the factory setting.

Selection guide within the Research plus range

The volume ranges vary significantly across the variable Research-plus models. The following overview shows neighbouring variable models from the same range:

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

Comparing Research plus, Reference 2 and Xplorer

Anyone choosing between the three Eppendorf manual pipette ranges should first consider the operation. The Research plus is adjusted via a mechanical adjustment wheel, with no electronics and no display, making it robust and low-maintenance. The Reference 2 replaces the adjustment wheel with a single-button operation featuring a digital display, which is practical for laboratories with frequently changing volumes and multiple users sharing the same device. The Xplorer and Xplorer Plus go one step further, pipetting electronically programmed sequences, with password protection included in the Plus version. For a clearly defined volume range in routine work, the mechanical Research Plus range remains the simplest choice.

Differentiation within the Research plus range

If the volume range of this pipette does not fully meet your requirements, it is worth considering neighbouring Research plus models, for example the Eppendorf Research plus single-channel pipette, variable (1–10 mL), 3123000080 Eppendorf Research plus G pipette, single-channel, variable, 2–20 µL, light grey, epT.I.P.S. box, 3123000098. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

The upper and lower sections can be autoclaved together at 121 °C and 1 bar overpressure for 20 minutes; it is not necessary to dismantle the lower section for this. The filter sleeve and protective filter are autoclaved separately. No re-lubrication is required after autoclaving; a gravimetric accuracy check is recommended afterwards. The pipette is stored without transport packaging at temperatures between –5 and 45 degrees Celsius and relative humidity of 10 to 95 per cent; during operation, the temperature range is 5 to 40 degrees Celsius within the same humidity range. Warranty claims are handled via the relevant Eppendorf authorised partner; claims are excluded for damage resulting from misuse, unauthorised opening of the upper section, and wear and tear on typical wear parts.

Fixed volume or variable setting

A variable-volume pipette is worthwhile whenever different volumes within the same range are required during a workflow, for example when carrying out dilution series or handling varying sample volumes. The adjustment wheel allows for adjustment without tools and without changing equipment. If, on the other hand, only a single volume is pipetted on a permanent basis, a fixed-volume pipette from the same range eliminates the risk of accidental misadjustment.

Frequently asked questions

Can the 100–1000 µL version be sterilised in an autoclave?
Yes, the upper and lower sections can both withstand 121 degrees Celsius, 1 bar overpressure and a dwell time of 20 minutes; it is not necessary to dismantle the lower section for this. The filter sleeve and protective filter are autoclaved separately.

How is accuracy checked after autoclaving?
Re-greasing is not required; however, Eppendorf still recommends a gravimetric check after autoclaving to confirm accuracy.

How is the volume set on the variable model?
Via the mechanical adjustment wheel on the pipette head, without a display or electronics. The set value is retained until the next adjustment.

Which pipette tips are compatible with the Eppendorf Research plus 100–1000 µL?
According to the operating instructions: epT.I.P.S. blue 71 mm (50–1000 µL).

Is the pipette an in vitro diagnostic device?
According to the operating instructions, the Research plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

Contents

• 1 Eppendorf Research plus single-channel pipette (100–1000 µL, blue)

Individual requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.