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Eppendorf Research plus single-channel pipette, variable, 2–20 µL, light grey, 3123000098

Product information "Eppendorf Research plus single-channel pipette, variable, 2–20 µL, light grey, 3123000098"

Varying sample volumes within the same volume range require a pipette that can be adjusted quickly and without the need for tools. The Eppendorf Research plus single-channel pipette, in its variable 2–20 µL version in light grey, meets precisely this need, with 0.02 µL increments and a spring-loaded tip cone that reduces the effort required to attach tips. It is designed for workstations where dilution series or different sample volumes are used in the same batch.

Typical applications

The Eppendorf Research plus 2–20 µL is suitable for, amongst other things:

  • PCR reactions and dilution series
  • Reagent addition in ELISA diagnostics
  • Sample preparation for sequencing
  • Pipetting small volumes into microtitre plates

Features and handling

A spring-loaded tip cone on all Research-plus models up to 1 mL ensures that pipette tips can be attached and ejected with minimal effort. The low weight of the Research-plus range is particularly noticeable in terms of ease of use during extensive pipetting runs. The lower section, featuring a Fortron PerfectPiston piston, can be quickly dismantled without tools for maintenance and cleaning.

For secondary adjustment – i.e. calibration for a liquid other than water or to a custom factory setting – the manufacturer states that Research-plus pipettes feature a user adjustment function that does not override the original factory setting.

Selection guide within the Research-plus range

The volume ranges vary significantly across the variable Research-plus models. The following overview shows neighbouring variable models from the same range:

VolumeColour codeMPN
1–10 mLturquoise3123000080
2–20 µLyellow3123000039
30–300 µLorange3123000101
100–1000 µLblue3123000063

The appropriate volume in each individual case depends on the actual sample quantities and reagent volumes involved in the respective step. Selecting a volume that is too large reduces relative accuracy at the lower end of the scale, whilst selecting a volume that is too small necessitates several pipetting steps instead of a single one.

Comparing Research plus, Reference 2 and Xplorer

Anyone choosing between the three Eppendorf manual pipette ranges should first consider the operation. The Research plus is adjusted via a mechanical adjustment wheel, with no electronics and no display, making it robust and low-maintenance. The Reference 2 replaces the adjustment wheel with a single-button operation featuring a digital display, which is practical for laboratories with frequently changing volumes and multiple users sharing the same device. The Xplorer and Xplorer Plus go one step further, pipetting electronically programmed sequences, including password protection on the Plus model. For a clearly defined volume range in routine work, the mechanical Research Plus range remains the simplest choice.

Differentiation within the Research plus range

If the volume range of this pipette does not fully meet your requirements, it is worth looking at neighbouring Research plus models, for example the Eppendorf Research plus single-channel pipette, variable (1–10 mL), 3123000080 Eppendorf Research plus G pipette, single-channel, variable, 2–20 µL, yellow, epT.I.P.S. box, 3123000039. This allows you to put together a suitable combination of several volume ranges without having to switch to a different product line.

Use, storage and maintenance

121 °C, 1 bar overpressure, 20 minutes: The pipette can be autoclaved whilst fully assembled; the lower section does not need to be dismantled for this – only the filter sleeve and protective filter are placed separately in the autoclave. There is no need to re-lubricate the pistons afterwards, although a gravimetric check is still advisable. Without transport packaging, the recommended storage conditions are between –5 and 45 degrees Celsius and 10 to 95 per cent relative humidity; during operation, the conditions are 5 to 40 degrees Celsius within the same humidity range. The relevant Eppendorf authorised partner is the point of contact for warranty matters, excluding wear parts and damage resulting from misuse or the unauthorised opening of the upper section.

Fixed volume or variable setting

A variable-volume pipette is worthwhile whenever different volumes within the same range are required during a workflow, such as for dilution series or varying sample volumes. The adjustment wheel allows for adjustment without tools and without changing equipment. If, on the other hand, only a single volume is pipetted on a permanent basis, a fixed-volume pipette from the same range eliminates the risk of accidental misadjustment.

Frequently asked questions

Is the Eppendorf Research plus 2–20 µL autoclavable?
Yes. The upper and lower sections can be autoclaved together at 121 degrees Celsius and 1 bar overpressure for 20 minutes without the lower section needing to be dismantled. The filter sleeve and protective filter are autoclaved separately.

How is accuracy checked after autoclaving?
Re-lubrication is not necessary; however, Eppendorf still recommends a gravimetric check after autoclaving to confirm accuracy.

How is the volume set on the variable model?
Via the mechanical adjustment wheel on the pipette head, without a display or electronics. The set value is retained until the next adjustment.

Which pipette tips are compatible with the Eppendorf Research plus 2–20 µL?
According to the operating instructions, epT.I.P.S. light grey 46 mm (0.5–20 µL).

Is the pipette an in vitro diagnostic device?
According to the operating instructions, the Research plus range is designed for the low-contamination transfer of liquids, including samples from the human body and reagents within the context of an in vitro diagnostic application as defined by Directive 98/79/EC.

To whom should warranty claims be addressed?
To the relevant Eppendorf authorised dealer. Excluded are damages resulting from misuse, unauthorised opening of the upper part, and wear and tear of typical wear parts.

Can the pipette be readjusted if it deviates from the factory setting?
According to the manufacturer, secondary calibration is provided for the Research-plus range. Details are set out in a separate Eppendorf calibration document, the full text of which was not available at the time of writing.

What does the IVD marking on the Research-plus range signify?
The user manual generically refers to the pipette as an in vitro diagnostic device within the meaning of Directive 98/79/EC, for the entire Research-plus range. No product-specific confirmation was available at the time of writing.

Technical details

RangeEppendorf Research plus
Modelvariable
Volume2–20 µL
Increment0.02 µL
Colour codelight grey
Compatible pipette tipepT.I.P.S. light grey 46 mm (0.5–20 µL)
Test volume 2 µLSystematic measurement error 5.0%/0.1 µLRandom measurement error 1.5%/0.03 µL
Test volume 10 µLSystematic measurement error: 1.2%/0.12 µLRandom measurement error: 0.6%/0.06 µL
Test volume 20 µLSystematic measurement error 1.0%/0.2 µLRandom measurement error 0.3%/0.06 µL
StandardTest in accordance with ISO 8655

Contents

  • 1 Eppendorf Research plus single-channel pipette (2–20 µL, light grey)

Individual requirements & procurement service

For suitable pipette tips, spare parts or additional volume ranges from the Research plus range, LT Laborhandel provides support via its own procurement service at lt-laborhandel.de/service.

Colour: Grey
Number of channels: 1-channel
Operation: mechanical
Volume range: 2 µl - 20 µl
Volume setting: variable