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Kern OCM 168 Inverted Microscope, Trinocular, 5 W LED for Reflected and Transmitted Light, Long Working Distance

Product information "Kern OCM 168 Inverted Microscope, Trinocular, 5 W LED for Reflected and Transmitted Light, Long Working Distance"

Cell cultures grow in dishes and plates, and anyone wishing to examine them needs space for the culture vessel above the objective lens. The Kern OCM 168 is an inverted microscope designed for this purpose: the objectives are positioned beneath the stage, the specimen rests on top, and, according to Kern, the working distance is very large. Kern describes the instrument as an inverted biological laboratory microscope with fluorescence capability. It features a trinocular head, Infinity-Plan objectives at 10x, 20x and 40x, a PH 20x phase-contrast objective, as well as LED transmitted light and LED reflected light of 5 watts each.

Equipment for culture monitoring

The mechanical stage travels 128 mm in one direction and 180 mm in the other. It is fitted with a specimen holder for Ø 110 mm. A holder for 54 mm culture dishes is included in the Scope of delivery; holders for 35 and 65 mm dishes are available from Kern as accessories. The Abbe condenser has an aperture of 0.3, an aperture diaphragm and a working distance of 72 mm. Kern specifies it for brightfield, phase contrast and fluorescence applications. A phase contrast slider, centring eyepiece and green filter are included as standard.

Lighting

The transmitted light source is a 5 W LED and is dimmable; the incident light source is a second 5 W LED for epi-illumination. Power is supplied via a plug-in power supply unit rated at 100 to 240 V, 50/60 Hz and 0.3 A (Protection Class III); the connection cable is detachable. The OCM 168 is constructed in the same way as the OCM 167, but features a fluorescence filter unit for UV, V, B and G instead of just B and G, and weighs 17 kg instead of 16 kg.

Head and eyepieces

The eyepieces are HWF 10x with a 22 mm field of view, anti-fungal coating and high eye point. The Siedentopf tube is trinocular and inclined at 45 degrees; the interpupillary distance can be adjusted from 48 to 76 mm, and dioptre adjustment is available on both sides. The coaxial coarse and fine adjustment mechanisms have a minimum step of 0.002 mm. With the 10x eyepieces, this results in a total magnification of 100x to 400x. A centring eyepiece is included as standard; an adjustable 10x eyepiece with a scale (0.1 mm) is available as an accessory.

Capturing images

The third tube outlet accommodates a camera. Kern offers a choice of the ODC 854 4K camera (HDMI and USB, Sony sensor), the ODC 251 HD tablet with 4K camera, the ODC 861 cooled camera for fluorescence, the ODC 825 and 832 cameras, and the ODC 241 tablet camera. The corresponding adapters are: C-mount with 0.5x magnification and adjustable focus (OBB-A1515), 0.75x (OBB-A1590) or the standard adapter for trinocular models (OBB-A1514).

Which OCM

The OCM 161 features halogen transmitted light without incident light, whilst the OCM 166 also features a 100 W HBO incident light. Like the OCM 167, the OCM 168 is an LED unit with incident and transmitted light, but features an extended filter set comprising UV, V, B and G.

Typical applications

Kern cites the observation and analysis of cell cultures as the primary applications for the OCM series.

  • Observation and analysis of cell cultures
  • Inspection in culture dishes on the mechanical stage
  • Brightfield, phase contrast and fluorescence

Frequently asked questions

Can I mount culture dishes? Yes. The specimen holder has a diameter of 110 mm; a holder for 54 mm dishes is included, whilst holders for 35 and 65 mm dishes are available as accessories.

What is the travel range of the stage? 128 mm and 180 mm.

What do I need for phase contrast? The PH 20x objective, the phase contrast sliders and a centring eyepiece are included.

What is the maximum total magnification? 100x to 400x using the 10x, 20x and 40x objectives and the 10x eyepieces.

Technical details

DesignInverted, infinity
TubeTrinocular, Siedentopf, inclined at 45 degrees
EyepiecesHWF 10x, 22 mm field of view, anti-fungus, high eye point
Interpupillary distance48 to 76 mm
Dioptre adjustmenton both sides
ObjectivesInfinity Plan 10x, 20x, 40x, PH 20x
Objective turret5 positions
CondenserAbbe, aperture 0.3, fixed, aperture diaphragm, working distance 72 mm
Illumination5 W LED transmitted light (dimmable), 5 W LED reflected light
StageMechanical, travel range 128 mm and 180 mm, specimen holder Ø 110 mm
FocusingCoaxial coarse and fine adjustment, fine adjustment 0.002 mm
Power supplyMains adaptor, 100 to 240 V, 50/60 Hz, 0.3 A, protection class III
Dimensions (W x D x H)782 x 304 x 530 mm
Weight (net)17 kg
Storage temperature-5 to 40 °C

Scope of delivery

  • OCM 168 inverted microscope
  • Mechanical stage with specimen holder (Ø 110 mm) and stage extension
  • Holder for 54 mm culture dishes
  • Phase contrast unit (slider, centring eyepiece, green filter)
  • Blue filter
  • Eyecups
  • Dust cover
  • Operating instructions

Individual requirements and procurement service

We can source additional culture dish holders, cameras, adaptors or lenses for the OCM 168 on request via our procurement service.